Summary
This report contains visualisations of statistics that can help in understanding the results from the wf-alignment workflow. Each section contains different plots or tables, and in general the results are broken down by sample or the reference file to which alignments were made. You can quickly jump to an individual section with the links in the header bar.
2 samples:barcode01 barcode02
2 reference files:
ERCC.fasta SIRV_isoforms_multi-fasta_170612a.fasta
99 reference sequences:
ERCC-00002 ERCC-00003 ERCC-00004 ERCC-00009 ERCC-00012 ERCC-00013 ERCC-00014 ...
Metric | Value | Percentage |
---|---|---|
Detected reference sequences | 29 |
|
Reads | 3,999 |
|
Reads aligned to 'ERCC.fasta' | 351 |
|
Reads aligned to 'SIRV_isoforms_multi-fasta_170612a.fasta' | 3,428 |
|
Unmapped reads | 220 |
|
Bases | 3,201,731 |
|
Metric | Value | Percentage |
---|---|---|
Detected reference sequences | 27 |
|
Reads | 1,600 |
|
Reads aligned to 'ERCC.fasta' | 225 |
|
Reads aligned to 'SIRV_isoforms_multi-fasta_170612a.fasta' | 1,250 |
|
Unmapped reads | 125 |
|
Bases | 1,252,199 |
|
Metric | Value | Percentage |
---|---|---|
Detected reference sequences | 10 |
|
Reads | 2,399 |
|
Reads aligned to 'ERCC.fasta' | 126 |
|
Reads aligned to 'SIRV_isoforms_multi-fasta_170612a.fasta' | 2,178 |
|
Unmapped reads | 95 |
|
Bases | 1,949,532 |
|
Reads Summary
Mean read quality
Per sample:
Per reference file:
Alignment accuracy
Per sample:
Per reference file:
Read coverage
These histograms show how much of an individual aligned read was covered by the alignment.
Per sample:
Per reference file:
Depth of coverage
This section illustrates the depth of coverage of the reference genomes. The left plot shows coverage vs. genomic position (note that the coordinates on the x-axis are the positions along the concatenated reference including all reference sequences in the respective reference file). The right plot shows the cumulative fraction of the reference that was covered to at least a certain depth.
Read count control
When a file with expected read counts was provided, this plot shows the observed vs. expected counts for each sample / reference sequence combination.