Sample status

This table gives the status of each sample, either completed successfully or the reason the workflow failed. If applicable the mean quality for the whole plasmid assembly has been provided, derived by Medaka from aligning the reads to the consensus.

The assembly was generated using Flye.
Sample pass/failed reason Length Mean Quality
sample01 Completed successfully 3036 41.21
sample02 Completed successfully 3037 37.4
sample03 Completed successfully 3096 35.71
sample04 Completed successfully 3096 36.83
sample05 Completed successfully 3036 42.6
sample06 Completed successfully 3036 41.21
sample07 Completed successfully 3096 39.3
sample08 Completed successfully 3096 38.95
sample09 Completed successfully 3036 35.42
sample10 Completed successfully 3036 35.98
sample11 Completed successfully 3096 33.32
sample12 Completed successfully 3096 41.04

Plannotate

The Plasmid annotation plot and feature table are produced using pLannotate

A pLannotate plot is shown for each assembly. A feature table provides descriptions of the annotated sequence. Unfilled features on the plannotate plots are incomplete features; the sequence match in the plasmid covers less than 95% of the full length of the feature in the database. These elements may be leftover fragments from earlier cloning steps used to create a plasmid. If they include only a small fraction of the feature, they likely do not still have the annotated function. However, even small feature fragments may affect plasmid function if they result in cryptic gene expression or are inadvertently combined with other elements during later cloning steps. The plannotate plot may have overlapping annotation labels, use the zoom and hover tools to decipher the labels.

Feature Database Identity Match Length Description Start Location End Location Length Strand
csgG swissprot 100.0% 100.0% CSGG_ECO57 - Experimental evidence at protein level: Swiss-Prot protein existence level 1. May be involved in the biogenesis of curli organelles. From Escherichia coli O157:H7. 1120 1951 831 +
rhaB promoter snapgene 100.0% 100.0% promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) 938 1057 119 +
cat promoter snapgene 100.0% 100.0% promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase 751 842 91 -
KanR snapgene 99.4% 100.0% aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes 2971 751 816 -
CloDF13 ori snapgene 100.0% 92.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 2162 2844 682 -
T7 terminator snapgene 100.0% 100.0% transcription terminator for bacteriophage T7 RNA polymerase 1981 2029 48 +
kanMX snapgene 99.2% 57.9% yeast selectable marker conferring kanamycin resistance (Wach et al., 1994) 2971 721 786 -
tonB terminator snapgene 100.0% 100.0% bidirectional E. coli tonB-P14 transcription terminator 842 874 32 -
T3Te terminator snapgene 100.0% 100.0% phage T3 early transcription terminator 2126 2156 30 +
RNAI Rfam 100.0% 99.0% Accession: RF00106 - RNAI 2741 2844 103 +
CloDF13 ori snapgene 99.0% 13.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 2868 2965 98 -
PDK intron snapgene 100.0% 5.1% pdk; modified pyruvate orthophosphate dikinase intron from Flaveria trinervia 3.0 750 832 82 -
Feature Database Identity Match Length Description Start Location End Location Length Strand
csgG swissprot 100.0% 100.0% CSGG_ECO57 - Experimental evidence at protein level: Swiss-Prot protein existence level 1. May be involved in the biogenesis of curli organelles. From Escherichia coli O157:H7. 1732 2563 831 -
rhaB promoter snapgene 100.0% 100.0% promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) 2626 2745 119 -
cat promoter snapgene 100.0% 100.0% promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase 2841 2932 91 +
KanR snapgene 99.4% 100.0% aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes 2932 711 816 +
CloDF13 ori snapgene 100.0% 92.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 839 1521 682 +
T7 terminator snapgene 100.0% 100.0% transcription terminator for bacteriophage T7 RNA polymerase 1654 1702 48 -
kanMX snapgene 99.2% 57.9% yeast selectable marker conferring kanamycin resistance (Wach et al., 1994) 2962 711 786 +
tonB terminator snapgene 100.0% 100.0% bidirectional E. coli tonB-P14 transcription terminator 2809 2841 32 +
T3Te terminator snapgene 100.0% 100.0% phage T3 early transcription terminator 1527 1557 30 -
RNAI Rfam 100.0% 99.0% Accession: RF00106 - RNAI 837 940 103 -
CloDF13 ori snapgene 100.0% 13.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 717 815 98 +
PDK intron snapgene 100.0% 5.1% pdk; modified pyruvate orthophosphate dikinase intron from Flaveria trinervia 3.0 2851 2933 82 +
Feature Database Identity Match Length Description Start Location End Location Length Strand
TXL_EISFE swissprot 100.0% 100.0% Experimental evidence at protein level: Swiss-Prot protein existence level 1. Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes. Has hemolytic activity and is also cytotoxic to spermatozoa of some species of invertebrates and many species of vertebrates and to amphibian larvae, guinea pig polymorphonuclear leukocytes, chicken fibroblasts, normal spleen cells and various tumor cells. Is lethal for various species of reptiles, amphibian, birds and mammals. Induces smooth muscle contraction. It binds sphingomyelin and induces hemolysis in the same manner as lysenin-related protein 2, and is 10-fold more effective than lysenin-related protein 1. 2976 771 891 -
rhaB promoter snapgene 100.0% 100.0% promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) 834 953 119 -
cat promoter snapgene 100.0% 100.0% promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase 1049 1140 91 +
KanR snapgene 99.4% 100.0% aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes 1140 1956 816 +
CloDF13 ori snapgene 100.0% 92.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 2083 2765 682 +
T7 terminator snapgene 100.0% 100.0% transcription terminator for bacteriophage T7 RNA polymerase 2898 2946 48 -
kanMX snapgene 99.2% 57.9% yeast selectable marker conferring kanamycin resistance (Wach et al., 1994) 1170 1956 786 +
tonB terminator snapgene 100.0% 100.0% bidirectional E. coli tonB-P14 transcription terminator 1017 1049 32 +
T3Te terminator snapgene 100.0% 100.0% phage T3 early transcription terminator 2771 2801 30 -
RNAI Rfam 100.0% 99.0% Accession: RF00106 - RNAI 2081 2184 103 -
CloDF13 ori snapgene 99.0% 13.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 1962 2059 98 +
PDK intron snapgene 100.0% 5.1% pdk; modified pyruvate orthophosphate dikinase intron from Flaveria trinervia 3.0 1059 1141 82 +
Feature Database Identity Match Length Description Start Location End Location Length Strand
TXL_EISFE swissprot 100.0% 100.0% Experimental evidence at protein level: Swiss-Prot protein existence level 1. Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes. Has hemolytic activity and is also cytotoxic to spermatozoa of some species of invertebrates and many species of vertebrates and to amphibian larvae, guinea pig polymorphonuclear leukocytes, chicken fibroblasts, normal spleen cells and various tumor cells. Is lethal for various species of reptiles, amphibian, birds and mammals. Induces smooth muscle contraction. It binds sphingomyelin and induces hemolysis in the same manner as lysenin-related protein 2, and is 10-fold more effective than lysenin-related protein 1. 1697 2588 891 -
rhaB promoter snapgene 100.0% 100.0% promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) 2651 2770 119 -
cat promoter snapgene 100.0% 100.0% promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase 2866 2957 91 +
KanR snapgene 99.4% 100.0% aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes 2957 677 816 +
CloDF13 ori snapgene 100.0% 92.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 804 1486 682 +
T7 terminator snapgene 100.0% 100.0% transcription terminator for bacteriophage T7 RNA polymerase 1619 1667 48 -
kanMX snapgene 99.2% 57.9% yeast selectable marker conferring kanamycin resistance (Wach et al., 1994) 2987 677 786 +
tonB terminator snapgene 100.0% 100.0% bidirectional E. coli tonB-P14 transcription terminator 2834 2866 32 +
T3Te terminator snapgene 100.0% 100.0% phage T3 early transcription terminator 1492 1522 30 -
RNAI Rfam 100.0% 99.0% Accession: RF00106 - RNAI 802 905 103 -
CloDF13 ori snapgene 99.0% 13.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 683 780 98 +
PDK intron snapgene 100.0% 5.1% pdk; modified pyruvate orthophosphate dikinase intron from Flaveria trinervia 3.0 2876 2958 82 +
Feature Database Identity Match Length Description Start Location End Location Length Strand
csgG swissprot 100.0% 100.0% CSGG_ECO57 - Experimental evidence at protein level: Swiss-Prot protein existence level 1. May be involved in the biogenesis of curli organelles. From Escherichia coli O157:H7. 765 1596 831 -
rhaB promoter snapgene 100.0% 100.0% promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) 1659 1778 119 -
cat promoter snapgene 100.0% 100.0% promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase 1874 1965 91 +
KanR snapgene 99.4% 100.0% aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes 1965 2781 816 +
CloDF13 ori snapgene 100.0% 92.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 2908 554 682 +
T7 terminator snapgene 100.0% 100.0% transcription terminator for bacteriophage T7 RNA polymerase 687 735 48 -
kanMX snapgene 99.2% 57.9% yeast selectable marker conferring kanamycin resistance (Wach et al., 1994) 1995 2781 786 +
tonB terminator snapgene 100.0% 100.0% bidirectional E. coli tonB-P14 transcription terminator 1842 1874 32 +
T3Te terminator snapgene 100.0% 100.0% phage T3 early transcription terminator 560 590 30 -
RNAI Rfam 100.0% 99.0% Accession: RF00106 - RNAI 2906 3009 103 -
CloDF13 ori snapgene 99.0% 13.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 2787 2884 98 +
PDK intron snapgene 100.0% 5.1% pdk; modified pyruvate orthophosphate dikinase intron from Flaveria trinervia 3.0 1884 1966 82 +
Feature Database Identity Match Length Description Start Location End Location Length Strand
csgG swissprot 100.0% 100.0% CSGG_ECO57 - Experimental evidence at protein level: Swiss-Prot protein existence level 1. May be involved in the biogenesis of curli organelles. From Escherichia coli O157:H7. 2136 2967 831 -
rhaB promoter snapgene 100.0% 100.0% promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) 3030 113 119 -
cat promoter snapgene 100.0% 100.0% promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase 209 300 91 +
KanR snapgene 99.4% 100.0% aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes 300 1116 816 +
CloDF13 ori snapgene 100.0% 92.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 1243 1925 682 +
T7 terminator snapgene 100.0% 100.0% transcription terminator for bacteriophage T7 RNA polymerase 2058 2106 48 -
kanMX snapgene 99.2% 57.9% yeast selectable marker conferring kanamycin resistance (Wach et al., 1994) 330 1116 786 +
tonB terminator snapgene 100.0% 100.0% bidirectional E. coli tonB-P14 transcription terminator 177 209 32 +
T3Te terminator snapgene 100.0% 100.0% phage T3 early transcription terminator 1931 1961 30 -
RNAI Rfam 100.0% 99.0% Accession: RF00106 - RNAI 1241 1344 103 -
CloDF13 ori snapgene 99.0% 13.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 1122 1219 98 +
PDK intron snapgene 100.0% 5.1% pdk; modified pyruvate orthophosphate dikinase intron from Flaveria trinervia 3.0 219 301 82 +
Feature Database Identity Match Length Description Start Location End Location Length Strand
TXL_EISFE swissprot 100.0% 100.0% Experimental evidence at protein level: Swiss-Prot protein existence level 1. Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes. Has hemolytic activity and is also cytotoxic to spermatozoa of some species of invertebrates and many species of vertebrates and to amphibian larvae, guinea pig polymorphonuclear leukocytes, chicken fibroblasts, normal spleen cells and various tumor cells. Is lethal for various species of reptiles, amphibian, birds and mammals. Induces smooth muscle contraction. It binds sphingomyelin and induces hemolysis in the same manner as lysenin-related protein 2, and is 10-fold more effective than lysenin-related protein 1. 2976 771 891 -
rhaB promoter snapgene 100.0% 100.0% promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) 834 953 119 -
cat promoter snapgene 100.0% 100.0% promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase 1049 1140 91 +
KanR snapgene 99.4% 100.0% aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes 1140 1956 816 +
CloDF13 ori snapgene 100.0% 92.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 2083 2765 682 +
T7 terminator snapgene 100.0% 100.0% transcription terminator for bacteriophage T7 RNA polymerase 2898 2946 48 -
kanMX snapgene 99.2% 57.9% yeast selectable marker conferring kanamycin resistance (Wach et al., 1994) 1170 1956 786 +
tonB terminator snapgene 100.0% 100.0% bidirectional E. coli tonB-P14 transcription terminator 1017 1049 32 +
T3Te terminator snapgene 100.0% 100.0% phage T3 early transcription terminator 2771 2801 30 -
RNAI Rfam 100.0% 99.0% Accession: RF00106 - RNAI 2081 2184 103 -
CloDF13 ori snapgene 99.0% 13.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 1962 2059 98 +
PDK intron snapgene 100.0% 5.1% pdk; modified pyruvate orthophosphate dikinase intron from Flaveria trinervia 3.0 1059 1141 82 +
Feature Database Identity Match Length Description Start Location End Location Length Strand
TXL_EISFE swissprot 100.0% 100.0% Experimental evidence at protein level: Swiss-Prot protein existence level 1. Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes. Has hemolytic activity and is also cytotoxic to spermatozoa of some species of invertebrates and many species of vertebrates and to amphibian larvae, guinea pig polymorphonuclear leukocytes, chicken fibroblasts, normal spleen cells and various tumor cells. Is lethal for various species of reptiles, amphibian, birds and mammals. Induces smooth muscle contraction. It binds sphingomyelin and induces hemolysis in the same manner as lysenin-related protein 2, and is 10-fold more effective than lysenin-related protein 1. 430 1321 891 +
rhaB promoter snapgene 100.0% 100.0% promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) 248 367 119 +
cat promoter snapgene 100.0% 100.0% promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase 61 152 91 -
KanR snapgene 99.4% 100.0% aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes 2341 61 816 -
CloDF13 ori snapgene 100.0% 92.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 1532 2214 682 -
T7 terminator snapgene 100.0% 100.0% transcription terminator for bacteriophage T7 RNA polymerase 1351 1399 48 +
kanMX snapgene 99.2% 57.9% yeast selectable marker conferring kanamycin resistance (Wach et al., 1994) 2341 31 786 -
tonB terminator snapgene 100.0% 100.0% bidirectional E. coli tonB-P14 transcription terminator 152 184 32 -
T3Te terminator snapgene 100.0% 100.0% phage T3 early transcription terminator 1496 1526 30 +
RNAI Rfam 100.0% 99.0% Accession: RF00106 - RNAI 2111 2214 103 +
CloDF13 ori snapgene 99.0% 13.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 2238 2335 98 -
PDK intron snapgene 100.0% 5.1% pdk; modified pyruvate orthophosphate dikinase intron from Flaveria trinervia 3.0 60 142 82 -
Feature Database Identity Match Length Description Start Location End Location Length Strand
csgG swissprot 100.0% 100.0% CSGG_ECO57 - Experimental evidence at protein level: Swiss-Prot protein existence level 1. May be involved in the biogenesis of curli organelles. From Escherichia coli O157:H7. 2117 2948 831 -
rhaB promoter snapgene 100.0% 100.0% promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) 3011 94 119 -
cat promoter snapgene 100.0% 100.0% promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase 190 281 91 +
KanR snapgene 99.4% 100.0% aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes 281 1097 816 +
CloDF13 ori snapgene 100.0% 92.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 1224 1906 682 +
T7 terminator snapgene 100.0% 100.0% transcription terminator for bacteriophage T7 RNA polymerase 2039 2087 48 -
kanMX snapgene 99.2% 57.9% yeast selectable marker conferring kanamycin resistance (Wach et al., 1994) 311 1097 786 +
tonB terminator snapgene 100.0% 100.0% bidirectional E. coli tonB-P14 transcription terminator 158 190 32 +
T3Te terminator snapgene 100.0% 100.0% phage T3 early transcription terminator 1912 1942 30 -
RNAI Rfam 100.0% 99.0% Accession: RF00106 - RNAI 1222 1325 103 -
CloDF13 ori snapgene 99.0% 13.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 1103 1200 98 +
PDK intron snapgene 100.0% 5.1% pdk; modified pyruvate orthophosphate dikinase intron from Flaveria trinervia 3.0 200 282 82 +
Feature Database Identity Match Length Description Start Location End Location Length Strand
csgG swissprot 100.0% 100.0% CSGG_ECO57 - Experimental evidence at protein level: Swiss-Prot protein existence level 1. May be involved in the biogenesis of curli organelles. From Escherichia coli O157:H7. 2137 2968 831 -
rhaB promoter snapgene 100.0% 100.0% promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) 3031 114 119 -
cat promoter snapgene 100.0% 100.0% promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase 210 301 91 +
KanR snapgene 99.4% 100.0% aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes 301 1117 816 +
CloDF13 ori snapgene 100.0% 92.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 1244 1926 682 +
T7 terminator snapgene 100.0% 100.0% transcription terminator for bacteriophage T7 RNA polymerase 2059 2107 48 -
kanMX snapgene 99.2% 57.9% yeast selectable marker conferring kanamycin resistance (Wach et al., 1994) 331 1117 786 +
tonB terminator snapgene 100.0% 100.0% bidirectional E. coli tonB-P14 transcription terminator 178 210 32 +
T3Te terminator snapgene 100.0% 100.0% phage T3 early transcription terminator 1932 1962 30 -
RNAI Rfam 100.0% 99.0% Accession: RF00106 - RNAI 1242 1345 103 -
CloDF13 ori snapgene 99.0% 13.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 1123 1220 98 +
PDK intron snapgene 100.0% 5.1% pdk; modified pyruvate orthophosphate dikinase intron from Flaveria trinervia 3.0 220 302 82 +
Feature Database Identity Match Length Description Start Location End Location Length Strand
TXL_EISFE swissprot 100.0% 100.0% Experimental evidence at protein level: Swiss-Prot protein existence level 1. Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes. Has hemolytic activity and is also cytotoxic to spermatozoa of some species of invertebrates and many species of vertebrates and to amphibian larvae, guinea pig polymorphonuclear leukocytes, chicken fibroblasts, normal spleen cells and various tumor cells. Is lethal for various species of reptiles, amphibian, birds and mammals. Induces smooth muscle contraction. It binds sphingomyelin and induces hemolysis in the same manner as lysenin-related protein 2, and is 10-fold more effective than lysenin-related protein 1. 2976 771 891 -
rhaB promoter snapgene 100.0% 100.0% promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) 834 953 119 -
cat promoter snapgene 100.0% 100.0% promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase 1049 1140 91 +
KanR snapgene 99.4% 100.0% aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes 1140 1956 816 +
CloDF13 ori snapgene 100.0% 92.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 2083 2765 682 +
T7 terminator snapgene 100.0% 100.0% transcription terminator for bacteriophage T7 RNA polymerase 2898 2946 48 -
kanMX snapgene 99.2% 57.9% yeast selectable marker conferring kanamycin resistance (Wach et al., 1994) 1170 1956 786 +
tonB terminator snapgene 100.0% 100.0% bidirectional E. coli tonB-P14 transcription terminator 1017 1049 32 +
T3Te terminator snapgene 100.0% 100.0% phage T3 early transcription terminator 2771 2801 30 -
RNAI Rfam 100.0% 99.0% Accession: RF00106 - RNAI 2081 2184 103 -
CloDF13 ori snapgene 99.0% 13.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 1962 2059 98 +
PDK intron snapgene 100.0% 5.1% pdk; modified pyruvate orthophosphate dikinase intron from Flaveria trinervia 3.0 1059 1141 82 +
Feature Database Identity Match Length Description Start Location End Location Length Strand
TXL_EISFE swissprot 100.0% 100.0% Experimental evidence at protein level: Swiss-Prot protein existence level 1. Pore-forming toxin that defensively acts against parasitic microorganisms by forming pores in sphingomyelin-containing membranes. Has hemolytic activity and is also cytotoxic to spermatozoa of some species of invertebrates and many species of vertebrates and to amphibian larvae, guinea pig polymorphonuclear leukocytes, chicken fibroblasts, normal spleen cells and various tumor cells. Is lethal for various species of reptiles, amphibian, birds and mammals. Induces smooth muscle contraction. It binds sphingomyelin and induces hemolysis in the same manner as lysenin-related protein 2, and is 10-fold more effective than lysenin-related protein 1. 304 1195 891 +
rhaB promoter snapgene 100.0% 100.0% promoter of the E. coli rhaBAD operon, conferring tight induction with L-rhamnose and repression with D-glucose in the presence of RhaR and RhaS (Giacalone et al., 2006) 122 241 119 +
cat promoter snapgene 100.0% 100.0% promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase 3031 26 91 -
KanR snapgene 99.4% 100.0% aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes 2215 3031 816 -
CloDF13 ori snapgene 100.0% 92.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 1406 2088 682 -
T7 terminator snapgene 100.0% 100.0% transcription terminator for bacteriophage T7 RNA polymerase 1225 1273 48 +
kanMX snapgene 99.2% 57.9% yeast selectable marker conferring kanamycin resistance (Wach et al., 1994) 2215 3001 786 -
tonB terminator snapgene 100.0% 100.0% bidirectional E. coli tonB-P14 transcription terminator 26 58 32 -
T3Te terminator snapgene 100.0% 100.0% phage T3 early transcription terminator 1370 1400 30 +
RNAI Rfam 100.0% 99.0% Accession: RF00106 - RNAI 1985 2088 103 +
CloDF13 ori snapgene 99.0% 13.3% Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins. 2112 2209 98 -
PDK intron snapgene 100.0% 5.1% pdk; modified pyruvate orthophosphate dikinase intron from Flaveria trinervia 3.0 3030 16 82 -

Read Counts

Number of reads per sample.

Read stats

For each assembly, read length statistics and plots of quality before and after downsampling, and after host filtering if a host reference was provided.

Completed successfully

Read count

4830

Read count

578

Read count

204

Completed successfully

Read count

4830

Read count

583

Read count

205

Completed successfully

Read count

1334

Read count

603

Read count

197

Completed successfully

Read count

1334

Read count

541

Read count

204

Completed successfully

Read count

1334

Read count

608

Read count

216

Completed successfully

Read count

1334

Read count

521

Read count

220

Completed successfully

Read count

1334

Read count

593

Read count

212

Completed successfully

Read count

1334

Read count

671

Read count

206

Completed successfully

Read count

1334

Read count

437

Read count

209

Completed successfully

Read count

1334

Read count

416

Read count

213

Completed successfully

Read count

1334

Read count

527

Read count

209

Completed successfully

Read count

1334

Read count

697

Read count

202

Insert sequences

This table shows which primers were found in the consensus sequence of each sample and where the inserts were found.

Sample start end primer strand Insert length
sample01 1061 2190 pRham + 1129
sample02 1493 2622 pRham - 1129
sample03 2737 830 pRham - 1189
sample04 1458 2647 pRham - 1189
sample05 526 1655 pRham - 1129
sample06 1897 3026 pRham - 1129
sample07 2737 830 pRham - 1189
sample08 371 1560 pRham + 1189
sample09 1878 3007 pRham - 1129
sample10 1898 3027 pRham - 1129
sample11 2737 830 pRham - 1189
sample12 245 1434 pRham + 1189

Multiple Sequence Alignment

This section shows the inserts aligned with each other or a reference sequence if provided. 

Reference GGCCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTATAAGAAGGAGATATACATATGCAGCGTCTGTTTCTGCTG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GTCGCGGTGGTGCTGCTGGCGGTTGTCTGACCGCACCGCCGAAAGAAGCGGCACGTCCGACCCTGATGCGTGCACAGTCT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TATAAAGATCTGACCCATCTGCCGGCTCCGACGGGCAAAATTTTGTTAGCGTCTATAACATCCAGGACGAAACCGGTCAA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TTTAAACCGTACCCGGCGAGTAATTTCTCCACAGCCGTTCCGCAGAGTGCAACCGCTATGCTGGTCACGGCACTGAAAGA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TTCCGTTGGTTCATTCCGCTGGAACGCCAGGGCCTGCAAAACCCTGCTGAATGAACGTAAAATTATCCGCGCAGCTCAGG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference AAAACAGTACCGTGGCCATTAACAATCGTATTCCGCTGCAAAGCCTGACCGCCGCAAACATCATGGTTGAAGGCTCTATC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ATCGGTTACAGATCAAACGTCAAATCGGGCGGTGTGGGCGCACGTTATTTTGGCATTGGTGCTGATACCCAGTACCAACT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GGACCAGATCCTGGTATGCGCGTTCCAGTTATCAGGTAGGTGTTTAACCAAGAAAGTTGTCAGTGTCTTTGTGGTTTTCG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CATTTATCGTGAAACGCTTTCGCGTTTTCGTGCGCCGCTTCACTGGGAGTTCGCAGAGGATTTGTTTAGCTAAACACGCG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GTTGCTCTTGAAGTGTGCGCCAAAGTCCGGCTACACTGGAAGGACAGATTTGGTTGCTGTGCTCTGCGAAAGCCAGTTAC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CACGGTTAAGCAGTTCCCCAACTGACTTAACCTTCGATCAAACCACCTCCCCAGGTGGTTTTTTCGAAGGTTAAGTCAGT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TGAGGAACTGCTTAACCTTCGATCAAACCACCTCCCCAGGTGGTTTTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference AAAGGATCTCAAGAAGATCCTTTGATCGCTAGCTTAGAAAAACTCATCGAGCATCAAGTGAAACTGCAATTTATTCATAT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCACCAACCAAACCGTTATTCATTCGTGATTGCGCCTGA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GCGAGACGAAATACGCGATCGCCGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCTGGGATCGCAGTGGTGA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGCCTG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference AATTTAATCGCGGCCTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAGCTCCTGAAAATCTCGATAACTCAAAAAAT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAAGTCAAAAGCCTCCGGTCGG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference AGGCTTTTGACTTTCTGCTATGGAGGTCAGGTATGATTTAAATGGTCAGTATTGAGCGATATCTAGAGAATTCGTCCACC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ACAATTCAGCAAATTGTGAACATCATCACGTTCATCTTTCCCTGGTTGCCAATGGCCCATTTTCCTGTCAGTAACGAGAA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GGTCGCGAATTCAGGCGCTTTTTAGACTGGTCGTAGGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTA
sample01  ---------------------------------------GACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTA
sample02  ---------------------------------------GACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTA
sample03  --------------------------------------------------------------------------------
sample04  ---------------------------------------GACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTA
sample05  ---------------------------------------GACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTA
sample06  ---------------------------------------GACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTA
sample07  --------------------------------------------------------------------------------
sample08  ---------------------------------------GACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTA
sample09  ---------------------------------------GACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTA
sample10  ---------------------------------------GACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTA
sample11  --------------------------------------------------------------------------------
sample12  ---------------------------------------GACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTA

Reference TAAGAAGGAGATATACATATG-CAGCGTCTGTTTCTGCTGGT--CGCGGTGAT-GCTGCTGA-GTGGTTGTCTGACCGCA
sample01  TAAGAAGGAGATATACATATG-CAGCGTCTGTTTCTGCTGGT--CGCGGTGAT-GCTGCTGA-GTGGTTGTCTGACCGCA
sample02  TAAGAAGGAGATATACATATG-CAGCGTCTGTTTCTGCTGGT--CGCGGTGAT-GCTGCTGA-GTGGTTGTCTGACCGCA
sample03  --------------------------------------------------------------------------------
sample04  TAAGAAGGAGATATACATATGAGTGCGAAGGCTGCTGAAGGTTATGAACAAATCGAAGTTGATGTGGTTG-CTGTGTGGA
sample05  TAAGAAGGAGATATACATATG-CAGCGTCTGTTTCTGCTGGT--CGCGGTGAT-GCTGCTGA-GTGGTTGTCTGACCGCA
sample06  TAAGAAGGAGATATACATATG-CAGCGTCTGTTTCTGCTGGT--CGCGGTGAT-GCTGCTGA-GTGGTTGTCTGACCGCA
sample07  --------------------------------------------------------------------------------
sample08  TAAGAAGGAGATATACATATGAGTGCGAAGGCTGCTGAAGGTTATGAACAAATCGAAGTTGATGTGGTTG-CTGTGTGGA
sample09  TAAGAAGGAGATATACATATG-CAGCGTCTGTTTCTGCTGGT--CGCGGTGAT-GCTGCTGA-GTGGTTGTCTGACCGCA
sample10  TAAGAAGGAGATATACATATG-CAGCGTCTGTTTCTGCTGGT--CGCGGTGAT-GCTGCTGA-GTGGTTGTCTGACCGCA
sample11  --------------------------------------------------------------------------------
sample12  TAAGAAGGAGATATACATATGAGTGCGAAGGCTGCTGAAGGTTATGAACAAATCGAAGTTGATGTGGTTG-CTGTGTGGA

Reference CCGCCG---A-----AAGAA----GCG---GCACGTCC---GACCCTG--ATGCCGCGTGCAC---AGTCTTATAAAGAT
sample01  CCGCCG---A-----AAGAA----GCG---GCACGTCC---GACCCTG--ATGCCGCGTGCAC---AGTCTTATAAAGAT
sample02  CCGCCG---A-----AAGAA----GCG---GCACGTCC---GACCCTG--ATGCCGCGTGCAC---AGTCTTATAAAGAT
sample03  --------------------------------------------------------------------------------
sample04  AGGAAGGTTATGTGTATGAAAATCGTGGTAGTACCTCCGTGGATCAAAAAATTAC-CAT-CACGAAAGGC--ATGAAGAA
sample05  CCGCCG---A-----AAGAA----GCG---GCACGTCC---GACCCTG--ATGCCGCGTGCAC---AGTCTTATAAAGAT
sample06  CCGCCG---A-----AAGAA----GCG---GCACGTCC---GACCCTG--ATGCCGCGTGCAC---AGTCTTATAAAGAT
sample07  --------------------------------------------------------------------------------
sample08  AGGAAGGTTATGTGTATGAAAATCGTGGTAGTACCTCCGTGGATCAAAAAATTAC-CAT-CACGAAAGGC--ATGAAGAA
sample09  CCGCCG---A-----AAGAA----GCG---GCACGTCC---GACCCTG--ATGCCGCGTGCAC---AGTCTTATAAAGAT
sample10  CCGCCG---A-----AAGAA----GCG---GCACGTCC---GACCCTG--ATGCCGCGTGCAC---AGTCTTATAAAGAT
sample11  --------------------------------------------------------------------------------
sample12  AGGAAGGTTATGTGTATGAAAATCGTGGTAGTACCTCCGTGGATCAAAAAATTAC-CAT-CACGAAAGGC--ATGAAGAA

Reference C-TGA----C-CCATCTG-CCGG-CTCCGACG-GGCAAAATTTTTGTTAGC-GT-CTATAACATCCAG-GAC------GA
sample01  C-TGA----C-CCATCTG-CCGG-CTCCGACG-GGCAAAATTTTTGTTAGC-GT-CTATAACATCCAG-GAC------GA
sample02  C-TGA----C-CCATCTG-CCGG-CTCCGACG-GGCAAAATTTTTGTTAGC-GT-CTATAACATCCAG-GAC------GA
sample03  --------------------------------------------------------------------------------
sample04  CGTTAATAGCGAAACCCGTACGGTCACCG-CGACGCA----TTCTATTGGCAGTACCATCTCCACGGGTGACGCCTTTGA
sample05  C-TGA----C-CCATCTG-CCGG-CTCCGACG-GGCAAAATTTTTGTTAGC-GT-CTATAACATCCAG-GAC------GA
sample06  C-TGA----C-CCATCTG-CCGG-CTCCGACG-GGCAAAATTTTTGTTAGC-GT-CTATAACATCCAG-GAC------GA
sample07  --------------------------------------------------------------------------------
sample08  CGTTAATAGCGAAACCCGTACGGTCACCG-CGACGCA----TTCTATTGGCAGTACCATCTCCACGGGTGACGCCTTTGA
sample09  C-TGA----C-CCATCTG-CCGG-CTCCGACG-GGCAAAATTTTTGTTAGC-GT-CTATAACATCCAG-GAC------GA
sample10  C-TGA----C-CCATCTG-CCGG-CTCCGACG-GGCAAAATTTTTGTTAGC-GT-CTATAACATCCAG-GAC------GA
sample11  --------------------------------------------------------------------------------
sample12  CGTTAATAGCGAAACCCGTACGGTCACCG-CGACGCA----TTCTATTGGCAGTACCATCTCCACGGGTGACGCCTTTGA

Reference AACCGG-TC-----AA-TTT-AAA--C-CGTA-CC-CGGCGAGT---AA-TTTC--T--CC---ACGGCCGT-TCCG---
sample01  AACCGG-TC-----AA-TTT-AAA--C-CGTA-CC-CGGCGAGT---AA-TTTC--T--CC---ACGGCCGT-TCCG---
sample02  AACCGG-TC-----AA-TTT-AAA--C-CGTA-CC-CGGCGAGT---AA-TTTC--T--CC---ACGGCCGT-TCCG---
sample03  --------------------------------------------------------------------------------
sample04  AATCGGCTCCGTGGAAGTTTCATATTCGCATAGCCACGAAGAATCACAAGTTTCGATGACCGAAACGGAAGTCTACGAAT
sample05  AACCGG-TC-----AA-TTT-AAA--C-CGTA-CC-CGGCGAGT---AA-TTTC--T--CC---ACGGCCGT-TCCG---
sample06  AACCGG-TC-----AA-TTT-AAA--C-CGTA-CC-CGGCGAGT---AA-TTTC--T--CC---ACGGCCGT-TCCG---
sample07  --------------------------------------------------------------------------------
sample08  AATCGGCTCCGTGGAAGTTTCATATTCGCATAGCCACGAAGAATCACAAGTTTCGATGACCGAAACGGAAGTCTACGAAT
sample09  AACCGG-TC-----AA-TTT-AAA--C-CGTA-CC-CGGCGAGT---AA-TTTC--T--CC---ACGGCCGT-TCCG---
sample10  AACCGG-TC-----AA-TTT-AAA--C-CGTA-CC-CGGCGAGT---AA-TTTC--T--CC---ACGGCCGT-TCCG---
sample11  --------------------------------------------------------------------------------
sample12  AATCGGCTCCGTGGAAGTTTCATATTCGCATAGCCACGAAGAATCACAAGTTTCGATGACCGAAACGGAAGTCTACGAAT

Reference C-AGAGTG---CAAC-CGCTATGCTGGTCACGGC-A-CTGAAAGATTC-C-CGTTGGTTCATTCCGCTGGAACGCCAG-G
sample01  C-AGAGTG---CAAC-CGCTATGCTGGTCACGGC-A-CTGAAAGATTC-C-CGTTGGTTCATTCCGCTGGAACGCCAG-G
sample02  C-AGAGTG---CAAC-CGCTATGCTGGTCACGGC-A-CTGAAAGATTC-C-CGTTGGTTCATTCCGCTGGAACGCCAG-G
sample03  --------------------------------------------------------------------------------
sample04  CAAAAGTGATTGAACACACCATTACGATCCCGCCGACCTCGAAG-TTCACGCGCTGG--CA----GCT-GAACG-CAGAT
sample05  C-AGAGTG---CAAC-CGCTATGCTGGTCACGGC-A-CTGAAAGATTC-C-CGTTGGTTCATTCCGCTGGAACGCCAG-G
sample06  C-AGAGTG---CAAC-CGCTATGCTGGTCACGGC-A-CTGAAAGATTC-C-CGTTGGTTCATTCCGCTGGAACGCCAG-G
sample07  --------------------------------------------------------------------------------
sample08  CAAAAGTGATTGAACACACCATTACGATCCCGCCGACCTCGAAG-TTCACGCGCTGG--CA----GCT-GAACG-CAGAT
sample09  C-AGAGTG---CAAC-CGCTATGCTGGTCACGGC-A-CTGAAAGATTC-C-CGTTGGTTCATTCCGCTGGAACGCCAG-G
sample10  C-AGAGTG---CAAC-CGCTATGCTGGTCACGGC-A-CTGAAAGATTC-C-CGTTGGTTCATTCCGCTGGAACGCCAG-G
sample11  --------------------------------------------------------------------------------
sample12  CAAAAGTGATTGAACACACCATTACGATCCCGCCGACCTCGAAG-TTCACGCGCTGG--CA----GCT-GAACG-CAGAT

Reference GCCTGCAAAACCTGCTGA-A-TGAACGTAAAATTATCCGCGCAGCTCAGGAAAACGGTA--CCG-TGGCCATTAACAATC
sample01  GCCTGCAAAACCTGCTGA-A-TGAACGTAAAATTATCCGCGCAGCTCAGGAAAACGGTA--CCG-TGGCCATTAACAATC
sample02  GCCTGCAAAACCTGCTGA-A-TGAACGTAAAATTATCCGCGCAGCTCAGGAAAACGGTA--CCG-TGGCCATTAACAATC
sample03  --------------------------------------------------------------------------------
sample04  GTCGGC----GGTGCTGACATTGAA--TATATGTA-CC-TG-ATC-GATGAA---GTTACCCCGATTGGCGGTACGCAGA
sample05  GCCTGCAAAACCTGCTGA-A-TGAACGTAAAATTATCCGCGCAGCTCAGGAAAACGGTA--CCG-TGGCCATTAACAATC
sample06  GCCTGCAAAACCTGCTGA-A-TGAACGTAAAATTATCCGCGCAGCTCAGGAAAACGGTA--CCG-TGGCCATTAACAATC
sample07  --------------------------------------------------------------------------------
sample08  GTCGGC----GGTGCTGACATTGAA--TATATGTA-CC-TG-ATC-GATGAA---GTTACCCCGATTGGCGGTACGCAGA
sample09  GCCTGCAAAACCTGCTGA-A-TGAACGTAAAATTATCCGCGCAGCTCAGGAAAACGGTA--CCG-TGGCCATTAACAATC
sample10  GCCTGCAAAACCTGCTGA-A-TGAACGTAAAATTATCCGCGCAGCTCAGGAAAACGGTA--CCG-TGGCCATTAACAATC
sample11  --------------------------------------------------------------------------------
sample12  GTCGGC----GGTGCTGACATTGAA--TATATGTA-CC-TG-ATC-GATGAA---GTTACCCCGATTGGCGGTACGCAGA

Reference ------------------GTATTCCGC---TGCAAAGCCTGACCGCCGCAAACATCATGGTTGAAGGCTCTATCATC--G
sample01  ------------------GTATTCCGC---TGCAAAGCCTGACCGCCGCAAACATCATGGTTGAAGGCTCTATCATC--G
sample02  ------------------GTATTCCGC---TGCAAAGCCTGACCGCCGCAAACATCATGGTTGAAGGCTCTATCATC--G
sample03  CGATTGGCGGTACGCAGAGTATTCCGCAAGTG-ATCACCT-CCCG-TGCAAAAATTATCGTTGGTCGCCAGATTATCCTG
sample04  ------------------GTATTCCGCAAGTG-ATCACCT-CCCG-TGCAAAAATTATCGTTGGTCGCCAGATTATCCTG
sample05  ------------------GTATTCCGC---TGCAAAGCCTGACCGCCGCAAACATCATGGTTGAAGGCTCTATCATC--G
sample06  ------------------GTATTCCGC---TGCAAAGCCTGACCGCCGCAAACATCATGGTTGAAGGCTCTATCATC--G
sample07  CGATTGGCGGTACGCAGAGTATTCCGCAAGTG-ATCACCT-CCCG-TGCAAAAATTATCGTTGGTCGCCAGATTATCCTG
sample08  ------------------GTATTCCGCAAGTG-ATCACCT-CCCG-TGCAAAAATTATCGTTGGTCGCCAGATTATCCTG
sample09  ------------------GTATTCCGC---TGCAAAGCCTGACCGCCGCAAACATCATGGTTGAAGGCTCTATCATC--G
sample10  ------------------GTATTCCGC---TGCAAAGCCTGACCGCCGCAAACATCATGGTTGAAGGCTCTATCATC--G
sample11  CGATTGGCGGTACGCAGAGTATTCCGCAAGTG-ATCACCT-CCCG-TGCAAAAATTATCGTTGGTCGCCAGATTATCCTG
sample12  ------------------GTATTCCGCAAGTG-ATCACCT-CCCG-TGCAAAAATTATCGTTGGTCGCCAGATTATCCTG

Reference GTTACGAATCAAACGTCAAATCGGGCGGTG-TGGGCGCACGTTAT-TTTGGCATTGGT--GCTG-ATACCCAGTACCAAC
sample01  GTTACGAATCAAACGTCAAATCGGGCGGTG-TGGGCGCACGTTAT-TTTGGCATTGGT--GCTG-ATACCCAGTACCAAC
sample02  GTTACGAATCAAACGTCAAATCGGGCGGTG-TGGGCGCACGTTAT-TTTGGCATTGGT--GCTG-ATACCCAGTACCAAC
sample03  GGCAAG-ACCGAAATTCGTATCAAAC-ATGCTGAACGCAAGGAATATATGACCGTGGTTAGCCGTAAATCTTG-GCCGGC
sample04  GGCAAG-ACCGAAATTCGTATCAAAC-ATGCTGAACGCAAGGAATATATGACCGTGGTTAGCCGTAAATCTTG-GCCGGC
sample05  GTTACGAATCAAACGTCAAATCGGGCGGTG-TGGGCGCACGTTAT-TTTGGCATTGGT--GCTG-ATACCCAGTACCAAC
sample06  GTTACGAATCAAACGTCAAATCGGGCGGTG-TGGGCGCACGTTAT-TTTGGCATTGGT--GCTG-ATACCCAGTACCAAC
sample07  GGCAAG-ACCGAAATTCGTATCAAAC-ATGCTGAACGCAAGGAATATATGACCGTGGTTAGCCGTAAATCTTG-GCCGGC
sample08  GGCAAG-ACCGAAATTCGTATCAAAC-ATGCTGAACGCAAGGAATATATGACCGTGGTTAGCCGTAAATCTTG-GCCGGC
sample09  GTTACGAATCAAACGTCAAATCGGGCGGTG-TGGGCGCACGTTAT-TTTGGCATTGGT--GCTG-ATACCCAGTACCAAC
sample10  GTTACGAATCAAACGTCAAATCGGGCGGTG-TGGGCGCACGTTAT-TTTGGCATTGGT--GCTG-ATACCCAGTACCAAC
sample11  GGCAAG-ACCGAAATTCGTATCAAAC-ATGCTGAACGCAAGGAATATATGACCGTGGTTAGCCGTAAATCTTG-GCCGGC
sample12  GGCAAG-ACCGAAATTCGTATCAAAC-ATGCTGAACGCAAGGAATATATGACCGTGGTTAGCCGTAAATCTTG-GCCGGC

Reference TG-GAC-C-AGATCGCAGTTAACCTGCGCGTGGTTAATGTCAGCACCG-GCGAA-ATTCTGAGCTCTGTGAATACCAGCA
sample01  TG-GAC-C-AGATCGCAGTTAACCTGCGCGTGGTTAATGTCAGCACCG-GCGAA-ATTCTGAGCTCTGTGAATACCAGCA
sample02  TG-GAC-C-AGATCGCAGTTAACCTGCGCGTGGTTAATGTCAGCACCG-GCGAA-ATTCTGAGCTCTGTGAATACCAGCA
sample03  GGCCACGCTGGGTCACAGTAAAC-------TGTTTAA-GTTCGTGCTGTACGAAGATT-GGGGCGGTTT---TCGCATCA
sample04  GGCCACGCTGGGTCACAGTAAAC-------TGTTTAA-GTTCGTGCTGTACGAAGATT-GGGGCGGTTT---TCGCATCA
sample05  TG-GAC-C-AGATCGCAGTTAACCTGCGCGTGGTTAATGTCAGCACCG-GCGAA-ATTCTGAGCTCTGTGAATACCAGCA
sample06  TG-GAC-C-AGATCGCAGTTAACCTGCGCGTGGTTAATGTCAGCACCG-GCGAA-ATTCTGAGCTCTGTGAATACCAGCA
sample07  GGCCACGCTGGGTCACAGTAAAC-------TGTTTAA-GTTCGTGCTGTACGAAGATT-GGGGCGGTTT---TCGCATCA
sample08  GGCCACGCTGGGTCACAGTAAAC-------TGTTTAA-GTTCGTGCTGTACGAAGATT-GGGGCGGTTT---TCGCATCA
sample09  TG-GAC-C-AGATCGCAGTTAACCTGCGCGTGGTTAATGTCAGCACCG-GCGAA-ATTCTGAGCTCTGTGAATACCAGCA
sample10  TG-GAC-C-AGATCGCAGTTAACCTGCGCGTGGTTAATGTCAGCACCG-GCGAA-ATTCTGAGCTCTGTGAATACCAGCA
sample11  GGCCACGCTGGGTCACAGTAAAC-------TGTTTAA-GTTCGTGCTGTACGAAGATT-GGGGCGGTTT---TCGCATCA
sample12  GGCCACGCTGGGTCACAGTAAAC-------TGTTTAA-GTTCGTGCTGTACGAAGATT-GGGGCGGTTT---TCGCATCA

Reference AAACGATCCTGTCTTACGAAGTGCAGGCTGGTGTTT--TTCGTTTCATTGAT-TATCAACGCCTGCTGGAAGGCGAAGTC
sample01  AAACGATCCTGTCTTACGAAGTGCAGGCTGGTGTTT--TTCGTTTCATTGAT-TATCAACGCCTGCTGGAAGGCGAAGTC
sample02  AAACGATCCTGTCTTACGAAGTGCAGGCTGGTGTTT--TTCGTTTCATTGAT-TATCAACGCCTGCTGGAAGGCGAAGTC
sample03  AAAC---CCTG-AATACGA--TGTATTCTGGTTATGAATACGCGT-ATAGCTCTGACCAGG---GC-GGTA-TCTACTTC
sample04  AAAC---CCTG-AATACGA--TGTATTCTGGTTATGAATACGCGT-ATAGCTCTGACCAGG---GC-GGTA-TCTACTTC
sample05  AAACGATCCTGTCTTACGAAGTGCAGGCTGGTGTTT--TTCGTTTCATTGAT-TATCAACGCCTGCTGGAAGGCGAAGTC
sample06  AAACGATCCTGTCTTACGAAGTGCAGGCTGGTGTTT--TTCGTTTCATTGAT-TATCAACGCCTGCTGGAAGGCGAAGTC
sample07  AAAC---CCTG-AATACGA--TGTATTCTGGTTATGAATACGCGT-ATAGCTCTGACCAGG---GC-GGTA-TCTACTTC
sample08  AAAC---CCTG-AATACGA--TGTATTCTGGTTATGAATACGCGT-ATAGCTCTGACCAGG---GC-GGTA-TCTACTTC
sample09  AAACGATCCTGTCTTACGAAGTGCAGGCTGGTGTTT--TTCGTTTCATTGAT-TATCAACGCCTGCTGGAAGGCGAAGTC
sample10  AAACGATCCTGTCTTACGAAGTGCAGGCTGGTGTTT--TTCGTTTCATTGAT-TATCAACGCCTGCTGGAAGGCGAAGTC
sample11  AAAC---CCTG-AATACGA--TGTATTCTGGTTATGAATACGCGT-ATAGCTCTGACCAGG---GC-GGTA-TCTACTTC
sample12  AAAC---CCTG-AATACGA--TGTATTCTGGTTATGAATACGCGT-ATAGCTCTGACCAGG---GC-GGTA-TCTACTTC

Reference GGTTA---CACC-TCAA-ACGAACCGGTGATG---C-TGTGTCTGATGTC-GGCGAT-TGAAACGG-G-TGT--T-ATTT
sample01  GGTTA---CACC-TCAA-ACGAACCGGTGATG---C-TGTGTCTGATGTC-GGCGAT-TGAAACGG-G-TGT--T-ATTT
sample02  GGTTA---CACC-TCAA-ACGAACCGGTGATG---C-TGTGTCTGATGTC-GGCGAT-TGAAACGG-G-TGT--T-ATTT
sample03  GATCAAGGCACCGACAACCCGAAACAGCGTTGGGCCATTAATAAGA-GCCTGCCGCTGCGCCATGGTGATGTCGTGACCT
sample04  GATCAAGGCACCGACAACCCGAAACAGCGTTGGGCCATTAATAAGA-GCCTGCCGCTGCGCCATGGTGATGTCGTGACCT
sample05  GGTTA---CACC-TCAA-ACGAACCGGTGATG---C-TGTGTCTGATGTC-GGCGAT-TGAAACGG-G-TGT--T-ATTT
sample06  GGTTA---CACC-TCAA-ACGAACCGGTGATG---C-TGTGTCTGATGTC-GGCGAT-TGAAACGG-G-TGT--T-ATTT
sample07  GATCAAGGCACCGACAACCCGAAACAGCGTTGGGCCATTAATAAGA-GCCTGCCGCTGCGCCATGGTGATGTCGTGACCT
sample08  GATCAAGGCACCGACAACCCGAAACAGCGTTGGGCCATTAATAAGA-GCCTGCCGCTGCGCCATGGTGATGTCGTGACCT
sample09  GGTTA---CACC-TCAA-ACGAACCGGTGATG---C-TGTGTCTGATGTC-GGCGAT-TGAAACGG-G-TGT--T-ATTT
sample10  GGTTA---CACC-TCAA-ACGAACCGGTGATG---C-TGTGTCTGATGTC-GGCGAT-TGAAACGG-G-TGT--T-ATTT
sample11  GATCAAGGCACCGACAACCCGAAACAGCGTTGGGCCATTAATAAGA-GCCTGCCGCTGCGCCATGGTGATGTCGTGACCT
sample12  GATCAAGGCACCGACAACCCGAAACAGCGTTGGGCCATTAATAAGA-GCCTGCCGCTGCGCCATGGTGATGTCGTGACCT

Reference TCCTGATCAATGATGGCA-TCGACCGTGGTCTGTGGGATCTGCAGAACAAAGCCGAACG-TCAAAATG-ACATTCTGGTG
sample01  TCCTGATCAATGATGGCA-TCGACCGTGGTCTGTGGGATCTGCAGAACAAAGCCGAACG-TCAAAATG-ACATTCTGGTG
sample02  TCCTGATCAATGATGGCA-TCGACCGTGGTCTGTGGGATCTGCAGAACAAAGCCGAACG-TCAAAATG-ACATTCTGGTG
sample03  TTATGAACAAATACTTCACGCGTTC-TGGTCTGTGCTAT-----GATGACGGCCCGGCGACCAATGTGTATTGTCTGGAT
sample04  TTATGAACAAATACTTCACGCGTTC-TGGTCTGTGCTAT-----GATGACGGCCCGGCGACCAATGTGTATTGTCTGGAT
sample05  TCCTGATCAATGATGGCA-TCGACCGTGGTCTGTGGGATCTGCAGAACAAAGCCGAACG-TCAAAATG-ACATTCTGGTG
sample06  TCCTGATCAATGATGGCA-TCGACCGTGGTCTGTGGGATCTGCAGAACAAAGCCGAACG-TCAAAATG-ACATTCTGGTG
sample07  TTATGAACAAATACTTCACGCGTTC-TGGTCTGTGCTAT-----GATGACGGCCCGGCGACCAATGTGTATTGTCTGGAT
sample08  TTATGAACAAATACTTCACGCGTTC-TGGTCTGTGCTAT-----GATGACGGCCCGGCGACCAATGTGTATTGTCTGGAT
sample09  TCCTGATCAATGATGGCA-TCGACCGTGGTCTGTGGGATCTGCAGAACAAAGCCGAACG-TCAAAATG-ACATTCTGGTG
sample10  TCCTGATCAATGATGGCA-TCGACCGTGGTCTGTGGGATCTGCAGAACAAAGCCGAACG-TCAAAATG-ACATTCTGGTG
sample11  TTATGAACAAATACTTCACGCGTTC-TGGTCTGTGCTAT-----GATGACGGCCCGGCGACCAATGTGTATTGTCTGGAT
sample12  TTATGAACAAATACTTCACGCGTTC-TGGTCTGTGCTAT-----GATGACGGCCCGGCGACCAATGTGTATTGTCTGGAT

Reference AAATACCGCCACATGAGTGTTCCACCAG-AATC--CTAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT
sample01  AAATACCGCCACATGAGTGTTCCACCAG-AATC--CTAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT
sample02  AAATACCGCCACATGAGTGTTCCACCAG-AATC--CTAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT
sample03  AAACGCGAAGACAAGTGGATTCTGGAAGTTGTCGGATAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT
sample04  AAACGCGAAGACAAGTGGATTCTGGAAGTTGTCGGATAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT
sample05  AAATACCGCCACATGAGTGTTCCACCAG-AATC--CTAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT
sample06  AAATACCGCCACATGAGTGTTCCACCAG-AATC--CTAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT
sample07  AAACGCGAAGACAAGTGGATTCTGGAAGTTGTCGGATAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT
sample08  AAACGCGAAGACAAGTGGATTCTGGAAGTTGTCGGATAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT
sample09  AAATACCGCCACATGAGTGTTCCACCAG-AATC--CTAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT
sample10  AAATACCGCCACATGAGTGTTCCACCAG-AATC--CTAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT
sample11  AAACGCGAAGACAAGTGGATTCTGGAAGTTGTCGGATAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT
sample12  AAACGCGAAGACAAGTGGATTCTGGAAGTTGTCGGATAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCT

Reference TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC
sample01  TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC
sample02  TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC
sample03  TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC
sample04  TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC
sample05  TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC
sample06  TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC
sample07  TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC
sample08  TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC
sample09  TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC
sample10  TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC
sample11  TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC
sample12  TGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATC

Reference ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC
sample01  ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC
sample02  ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC
sample03  ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC
sample04  ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC
sample05  ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC
sample06  ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC
sample07  ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC
sample08  ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC
sample09  ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC
sample10  ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC
sample11  ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC
sample12  ATTCAGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTC

Reference GGGATCCGCGCTGCGGACACATACAAAGTTACCCACAGATTCCGTGGATAAGCAGGGGACTAACATGTGAGGCAAAACAG
sample01  GGGATCCGCGCTGCGGACACATACAAAGTTACCCA---------------------------------------------
sample02  GGGATCCGCGCTGCGGACACATACAAAGTTACCCA---------------------------------------------
sample03  GGGATCCGCGCTGCGGACACATACAAAGTTACCCACAGATTCCGTGGATAAGCAGGGGACTAACATGTGAGGCAAAACAG
sample04  GGGATCCGCGCTGCGGACACATACAAAGTTACCCA---------------------------------------------
sample05  GGGATCCGCGCTGCGGACACATACAAAGTTACCCA---------------------------------------------
sample06  GGGATCCGCGCTGCGGACACATACAAAGTTACCCA---------------------------------------------
sample07  GGGATCCGCGCTGCGGACACATACAAAGTTACCCACAGATTCCGTGGATAAGCAGGGGACTAACATGTGAGGCAAAACAG
sample08  GGGATCCGCGCTGCGGACACATACAAAGTTACCCA---------------------------------------------
sample09  GGGATCCGCGCTGCGGACACATACAAAGTTACCCA---------------------------------------------
sample10  GGGATCCGCGCTGCGGACACATACAAAGTTACCCA---------------------------------------------
sample11  GGGATCCGCGCTGCGGACACATACAAAGTTACCCACAGATTCCGTGGATAAGCAGGGGACTAACATGTGAGGCAAAACAG
sample12  GGGATCCGCGCTGCGGACACATACAAAGTTACCCA---------------------------------------------

Reference CAGGGCCGCGCCGGTGGCGTTTTTCCATAGGCTCCGCCCTCCTGCCAGAGTTCACATAAACAGACGCTTTTCCGGTGCAT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  CAGGGCCGCGCCGGTGGCGTTTTTCCATAGGCTCCGCCCTCCTGCCAGAGTTCACATAAACAGACGCTTTTCCGGTGCAT
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  CAGGGCCGCGCCGGTGGCGTTTTTCCATAGGCTCCGCCCTCCTGCCAGAGTTCACATAAACAGACGCTTTTCCGGTGCAT
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  CAGGGCCGCGCCGGTGGCGTTTTTCCATAGGCTCCGCCCTCCTGCCAGAGTTCACATAAACAGACGCTTTTCCGGTGCAT
sample12  --------------------------------------------------------------------------------

Reference CTGTGGGAGCCGTGAGGCTCAACCATGAATCTGACAGTACGGGCGAAACCCGACAGGACTTAAAGATCCCCACCGTTTCC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  CTGTGGGAGCCGTGAGGCTCAACCATGAATCTGACAGTACGGGCGAAACCCGACAGGACTTAAAGATCCCCACCGTTTCC
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  CTGTGGGAGCCGTGAGGCTCAACCATGAATCTGACAGTACGGGCGAAACCCGACAGGACTTAAAGATCCCCACCGTTTCC
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  CTGTGGGAGCCGTGAGGCTCAACCATGAATCTGACAGTACGGGCGAAACCCGACAGGACTTAAAGATCCCCACCGTTTCC
sample12  --------------------------------------------------------------------------------

Reference GGCGGGTCGCTCCCTCTTGCGCTCTCCTGTTCCGACCCTGCCGTTTACCGGATACCTGTTCCGCCTTTCTCCCTTACGGG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  GGCGGGTCGCTCCCTCTTGCGCTCTCCTGTTCCGACCCTGCCGTTTACCGGATACCTGTTCCGCCTTTCTCCCTTACGGG
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  GGCGGGTCGCTCCCTCTTGCGCTCTCCTGTTCCGACCCTGCCGTTTACCGGATACCTGTTCCGCCTTTCTCCCTTACGGG
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  GGCGGGTCGCTCCCTCTTGCGCTCTCCTGTTCCGACCCTGCCGTTTACCGGATACCTGTTCCGCCTTTCTCCCTTACGGG
sample12  --------------------------------------------------------------------------------

Reference AAGTGTGGCGCTTTCTCATAGCTCACACACTGGTATCTCGGCTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTAAGCA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  AAGTGTGGCGCTTTCTCATAGCTCACACACTGGTATCTCGGCTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTAAGCA
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  AAGTGTGGCGCTTTCTCATAGCTCACACACTGGTATCTCGGCTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTAAGCA
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  AAGTGTGGCGCTTTCTCATAGCTCACACACTGGTATCTCGGCTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTAAGCA
sample12  --------------------------------------------------------------------------------

Reference AGAACTCCCCGTTCAGCCCGACTGCTGCGCCTTATCCGGTAACTGTTCACTTGAGTCCAACCCGGAAAAGCACGGTAAAA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  AGAACTCCCCGTTCAGCCCGACTGCTGCGCCTTATCCGGTAACTGTTCACTTGAGTCCAACCCGGAAAAGCACGGTAAAA
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  AGAACTCCCCGTTCAGCCCGACTGCTGCGCCTTATCCGGTAACTGTTCACTTGAGTCCAACCCGGAAAAGCACGGTAAAA
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  AGAACTCCCCGTTCAGCCCGACTGCTGCGCCTTATCCGGTAACTGTTCACTTGAGTCCAACCCGGAAAAGCACGGTAAAA
sample12  --------------------------------------------------------------------------------

Reference CGCCACTGGCAGCAGCCATTGGTAACTGGGAGTTCGCAGAGGATTTGTTTAGCTAAACACGCGGTTGCTCTTGAAGTGTG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  CGCCACTGGCAGCAGCCATTGGTAAC------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  CGCCACTGGCAGCAGCCATTGGTAAC------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  CGCCACTGGCAGCAGCCATTGGTAAC------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CGCCAAAGTCCGGCTACACTGGAAGGACAGATTTGGTTGCTGTGCTCTGCGAAAGCCAGTTACCACGGTTAAGCAGTTCC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CCAACTGACTTAACCTTCGATCAAACCACCTCCCCAGGTGGTTTTTTCGAAGGTTAAGTCAGTTGGGGAACTGCTTAACC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TTCGATCAAACCACCTCCCCAGGTGGTTTTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAAGGATCTCAAGAAGA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TCCTTTGATCGCTAGCTTAGAAAAACTCATCGAGCATCAAGTGAAACTGCAATTTATTCATATCAGGATTATCAATACCA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAAT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TTACGCTCGTCATCAAAATCACTCGCACCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ATCGCCGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCTGGGATCGCAGTGGTGAGTAACCATGCATCATCA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGCCTGACCATCTCATCTGTAAC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GAGCAAGACGTTTCCCGTTGAATATGGCTCATAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTTTCTG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CTATGGAGGTCAGGTATGATTTAAATGGTCAGTATTGAGCGATATCTAGAGAATTCGTCCACCACAATTCAGCAAATTGT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GAACATCATCACGTTCATCTTTCCCTGGTTGCCAATGGCCCATTTTCCTGTCAGTAACGAGAAGGTCGCGAATTCAGGCG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CTTTTTAGACTGGTCGTAGGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTATAAGAAGGAGATATACA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TATGCAGCGTCTGTTTCTGCTGGTCGCGGTGATGCTGCTGAGTGGTTGTCTGACCGCACCGCCGAAAGAAGCGGCACGTC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CGACCCTGATGCCGCGTGCACAGTCTTATAAAGATCTGACCCATCTGCCGGCTCCGACGGGCAAAATTTTTGTTAGCGTC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TATAACATCCAGGACGAAACCGGTCAATTTAAACCGTACCCGGCGAGTAATTTCTCCACGGCCGTTCCGCAGAGTGCAAC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CGCTATGCTGGTCACGGCACTGAAAGATTCCCGTTGGTTCATTCCGCTGGAACGCCAGGGCCTGCAAAACCTGCTGAATG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference AACGTAAAATTATCCGCGCAGCTCAGGAAAACGGTACCGTGGCCATTAACAATCGTATTCCGCTGCAAAGCCTGACCGCC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GCAAACATCATGGTTGAAGGCTCTATCATCGGTTACGAATCAAACGTCAAATCGGGCGGTGTGGGCGCACGTTATTTTGG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CATTGGTGCTGATACCCAGTACCAACTGGACCAGATCGCAGTTAACCTGCGCGTGGTTAATGTCAGCACCGGCGAAATTC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TGAGCTCTGTGAATACCAGCAAAACGATCCTGTCTTACGAAGTGCAGGCTGGTGTTTTTCGTTTCATTGATTATCAACGC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CTGCTGGAAGGCGAAGTCGGTTACACCTCAAACGAACCGGTGATGCTGTGTCTGATGTCGGCGATTGAAACGGGTGTTAT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TTTCCTGATCAATGATGGCATCGACCGTGGTCTGTGGGATCTGCAGAACAAAGCCGAACGTCAAAATGACATTCTGGTGA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference AATACCGCCACATGAGTGTTCCACCAGAATCCTAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATCATTC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference AGGACGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTCGGGA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TCCGCGCTGCGGACACATACAAAGTTACCCACAGATTCCGTGGATAAGCAGGGGACTAACATGTGAGGCAAAACAGCAGG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GCCGCGCCGGTGGCGTTTTTCCATAGGCTCCGCCCTCCTGCCAGAGTTCACATAAACAGACGCTTTTCCGGTGCATCTGT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GGGAGCCGTGAGGCTCAACCATGAATCTGACAGTACGGGCGAAACCCGACAGGACTTAAAGATCCCCACCGTTTCCGGCG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GGTCGCTCCCTCTTGCGCTCTCCTGTTCCGACCCTGCCGTTTACCGGATACCTGTTCCGCCTTTCTCCCTTACGGGAAGT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GTGGCGCTTTCTCATAGCTCACACACTGGTATCTCGGCTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTAAGCAAGAA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CTCCCCGTTCAGCTTGACTGCTGCGCCTTATCCGGTAACTGTTCACTTGAGTCCAACCCGGAAAAGCACGGTAAAACGCC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ACTGGCAGCAGCCATTGGTAACTGGGAGTTCGCAGAGGATTTGTTTAGCTAAACACGCGGTTGCTCTTGAAGTGTGCGCC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference AAAGTCCGGCTACACTGGAAGGACAGATTTGGTTGCTGTGCTCTGCGAAAGCCAGTTACCACGGTTAAGCAGTTCCCCAA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CTGACTTAACCTTCGATCAAACCACCTCCCCAGGTGGTTTTTTCGAAGGTTAAGTCAGTTGGGGAACTGCTTAACCTTCG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ATCAAACCACCTCCCCAGGTGGTTTTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAAGGATCTCAAGAAGATCCT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TTGATCGCTAGCTTAGAAAAACTCATCGAGCATCAAGTGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTAC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GCTCGTCATCAAAATCACTCGCACCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CCGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCTGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGCCTGACCATCTCATCTGTAACATCA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTCGAGC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference AAGACGTTTCCCGTTGAATATGGCTCATAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTTTCTGCTAT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GGAGGTCAGGTATGATTTAAATGGTCAGTATTGAGCGATATCTAGAGAATTCGTCCACCACAATTCAGCAAATTGTGAAC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ATCATCACGTTCATCTTTCCCTGGTTGCCAATGGCCCATTTTCCTGTCAGTAACGAGAAGGTCGCGAATTCAGGCGCTTT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TTAGACTGGTCGTAGGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTATAAGAAGGAGATATACATATG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CAGCGTCTGTTTCTGCTGGTCGCGGTGATGCTGCTGAGTGGTTGTCTGACCGCACCGCCGAAAGAAGCGGCACGTCCGAC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CCTGATGCCGCGTGCACAGTCTTATAAAGATCTGACCCATCTGCCGGCTCCGACGGGCAAAATTTTTGTTAGCGTCTATA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ACATCCAGGACGAAACCGGTCAATTTAAACCGTACCCGGCGAGTAATTTCTCCACGGCCGTTCCGCAGAGTGCAACCGCT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ATGCTGGTCACGGCACTGAAAGATTCCCGTTGGTTCATTCCGCTGGAACGCCAGGGCCTGCAAAACCTGCTGAATGAACG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TAAAATTATCCGCGCAGCTCAGGAAAACGGTACCGTGGCCATTAACAATCGTATTCCGCTGCAAAGCCTGACCGCCGCAA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference ACATCATGGTTGAAGGCTCTATCATCGGTTACGAATCAAACGTCAAATCGGGCGGTGTGGGCGCACGTTATTTTGGCATT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference GGTGCTGATACCCAGTACCAACTGGACCAGATCGCAGTTAACCTGCGCGTGGTTAATGTCAGCACCGGCGAAATTCTGAG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CTCTGTGAATACCAGCAAAACGATCCTGTCTTACGAAGTGCAGGCTGGTGTTTTTCGTTTCATTGATTATCAACGCCTGC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference TGGAAGGCGAAGTCGGTTACACCTCAAACGAACCGGTGATGCTGTGTCTGATGTCGGCGATTGAAACGGGTGTTATTTTC
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CTGATCAATGATGGCATCGACCGTGGTCTGTGGGATCTGCAGAACAAAGCCGAACGTCAAAATGACATTCTGGTGAAATA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CCGCCACATGAGTGTTCCACCAGAATCCTAATGAGCAGCAGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCT
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGTAACGAATTCAAGCTTGATATCATTCAGGA
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CGAGCCTCAGACTCCAGCGTAACTGGACTGCAATCAACTCACTGGCTCACCTTCACGGGTGGGCCTTTCTTCGGGATCCG
sample01  --------------------------------------------------------------------------------
sample02  --------------------------------------------------------------------------------
sample03  --------------------------------------------------------------------------------
sample04  --------------------------------------------------------------------------------
sample05  --------------------------------------------------------------------------------
sample06  --------------------------------------------------------------------------------
sample07  --------------------------------------------------------------------------------
sample08  --------------------------------------------------------------------------------
sample09  --------------------------------------------------------------------------------
sample10  --------------------------------------------------------------------------------
sample11  --------------------------------------------------------------------------------
sample12  --------------------------------------------------------------------------------

Reference CGCTGCGGACACATACAAAGTTACCCA
sample01  ---------------------------
sample02  ---------------------------
sample03  ---------------------------
sample04  ---------------------------
sample05  ---------------------------
sample06  ---------------------------
sample07  ---------------------------
sample08  ---------------------------
sample09  ---------------------------
sample10  ---------------------------
sample11  ---------------------------
sample12  ---------------------------

Insert variants

The following tables and figures are output from bcftools from finding any variants between the consensus insert and the provided reference insert.

Variant counts per sample. See output bcf file for info on individual variants.

filename id MNPs SNPs indels multiallelic SNP sites multiallelic sites no-ALTs others records
sample03.stats sample03.stats 0 0 0 0 0 0 0 0
sample09.stats sample09.stats 0 0 0 0 0 0 0 0
sample01.stats sample01.stats 0 0 0 0 0 0 0 0
sample07.stats sample07.stats 0 0 0 0 0 0 0 0
sample11.stats sample11.stats 0 0 0 0 0 0 0 0
sample12.stats sample12.stats 0 0 0 0 0 0 0 0
sample10.stats sample10.stats 0 0 0 0 0 0 0 0
sample08.stats sample08.stats 0 0 0 0 0 0 0 0
sample06.stats sample06.stats 0 0 0 0 0 0 0 0
sample02.stats sample02.stats 0 0 0 0 0 0 0 0
sample04.stats sample04.stats 0 0 0 0 0 0 0 0
sample05.stats sample05.stats 0 0 0 0 0 0 0 0

Trans counts per sample. See output bcf file for info on individual transitions.

filename id ts tv ts/tv ts (1st ALT) tv (1st ALT) ts/tv (1st ALT)
sample03.stats sample03.stats 0 0 0.0 0 0 0.0
sample09.stats sample09.stats 0 0 0.0 0 0 0.0
sample01.stats sample01.stats 0 0 0.0 0 0 0.0
sample07.stats sample07.stats 0 0 0.0 0 0 0.0
sample11.stats sample11.stats 0 0 0.0 0 0 0.0
sample12.stats sample12.stats 0 0 0.0 0 0 0.0
sample10.stats sample10.stats 0 0 0.0 0 0 0.0
sample08.stats sample08.stats 0 0 0.0 0 0 0.0
sample06.stats sample06.stats 0 0 0.0 0 0 0.0
sample02.stats sample02.stats 0 0 0.0 0 0 0.0
sample04.stats sample04.stats 0 0 0.0 0 0 0.0
sample05.stats sample05.stats 0 0 0.0 0 0 0.0

Dot plots

These dot plots have been created by aligning the assembly to itself to reveal any repeats or repetitive regions. Black is for repeats found in the forward strand and red for repeats found in the reverse complement. This was done using last

Software versions

Name Version
medaka 1.11.1
flye 2.9.2-b1786
minimap2 2.26-r1175
samtools 1.18
seqkit v2.5.1
Trycycler v0.5.4
bedtools v2.31.0
fastcat 0.14.1
rasusa 0.7.1
spoa 0.0.10
pandas 1.3.5
plannotate 1.2.0
bokeh 3.1.1

Workflow parameters

Key Value
help False
version False
out_dir wf-clone-validation
fastq clone_val_test/fastq
db_directory None
threads 4
host_reference clone_val_test/host_reference.fa.gz
regions_bedfile clone_val_test/reference.bed
approx_size 7000
assm_coverage 60
trim_length 150
prefix None
primers clone_val_test/primers.tsv
insert_reference clone_val_test/insert_reference.fasta
sample None
sample_sheet clone_val_test/sample_sheet.csv
disable_ping False
analyse_unclassified False
basecaller_cfg dna_r10.4.1_e8.2_400bps_sup@v4.2.0
medaka_model None
flye_quality nano-hq
non_uniform_coverage False
large_construct False
assembly_tool flye
canu_fast False
client_fields None
monochrome_logs False
validate_params True
show_hidden_params False
schema_ignore_params show_hidden_params,validate_params,monochrome_logs,aws_queue,aws_image_prefix,wf
wf {'example_cmd': ['--fastq wf-clone-validation-demo/test', '--primers wf-clone-validation-demo/primers.tsv'], 'container_sha': 'sha840e83632b197d53e8d182e5804ac8429f235d51', 'container_sha_medaka': 'sha61a9438d745a78030738352e084445a2db3daa2a', 'common_sha': 'sha1c5febff9f75143710826498b093d9769a5edbb9', 'container_sha_canu': 'shac3e8468ade9e2b47bf2c4d99ed3e6593a5100c1a', 'agent': 'cw-ci'}