EPI2ME 23.05-02 Release

By Stephen Rudd
Published in Software Releases
May 31, 2023
1 min read
EPI2ME 23.05-02 Release

Dear Nanopore Community,

With this post London Calling ‘23 bioinformatics product release, we are delighted to introduce the updated EPI2ME desktop application that was described by Chris Wright during his Data For Breakfast presentation - this is available online.

This update provides a simplified user interface to aid the entry of paths and parameters required by workflows (example shown in figure 1). Updates in the future will include new functionality to run analyses in the cloud. Please register your interest for this cloud functionality - we’ll soon be looking for test projects.

New look EPI2ME 5 Application
Figure 1.  EPI2ME v5.0.2 has an updated user interface. In the screenshot a user is prompted to provide the required information to run the metagenomics workflow, wf-metagenomics. The structuring of workflow options is simplified.

We are also pleased to introduce two new datasets that are now available through the s3://ont-open-data resource.

  • The Ashkenazi trio and HG001 (CEPH/Utah) have been sequenced from libraries prepared using ligation kit 14 and sequenced using the latest 5khz sampling rate - please see the Sequencing Genome in a Bottle samples blog post.
  • The COLO829 tumor/normal pair has been prepared using Ligation Sequencing Kit V14; a description of the data release can be found in the Sequencing A Tumor Normal Pair with Ligation Sequencing Kit V14 post - this dataset is a useful reference dataset to explore our recently introduced wf-somatic-variation workflow.

A couple of workflows have also been updated

  • wf-basecalling (v0.6.0) now works in real time - the workflow watches the input path and process raw data files as they become available. 
  • wf-transcriptomes (v0.1.12) has been updated to accommodate different reference annotation file types - annotations in both GFF3 and GTF format (optionally .gz compressed) are now accepted. 

We welcome all feedback and recommendations for new workflows and functionality. Please register your interest and we’ll tell you more about the upcoming cloud functionality.




Stephen Rudd

Stephen Rudd

Director, Bioinformatics Product

Related Posts

EPI2ME 24.06-01 Release
June 05, 2024
3 min

Quick Links

TutorialsWorkflowsOpen DataContact

Social Media

© 2020 - 2024 Oxford Nanopore Technologies plc. All rights reserved. Registered Office: Gosling Building, Edmund Halley Road, Oxford Science Park, OX4 4DQ, UK | Registered No. 05386273 | VAT No 336942382. Oxford Nanopore Technologies, the Wheel icon, EPI2ME, Flongle, GridION, Metrichor, MinION, MinIT, MinKNOW, Plongle, PromethION, SmidgION, Ubik and VolTRAX are registered trademarks of Oxford Nanopore Technologies plc in various countries. Oxford Nanopore Technologies products are not intended for use for health assessment or to diagnose, treat, mitigate, cure, or prevent any disease or condition.