We are happy to annouce the release of a Nanopore sequencing dataset of the Genome in a Bottle sample GM24385.
Multiple PromethION flowcells using both the R9.4.1 and R10.3 nanopores. The direct sequencer output is included, raw signal data stored in .fast5 files and basecalled data in .fastq file. Additional secondary analyses are included, notably alignments of sequence data to the reference genome are provided along with statistics derived from these.
The dataset comprises multiple R9.4.1 and R10.3 flowcells of multiple separately prepared samples; each sample was run on each flowcell type.
The initial sequencer outputs are included in self container directories. In addition derived outputs from an automated pipeline are stored separately.
Details concerning sample preparations
Below is described briefly the method of analyte preparation. Standard, published protocols were followed with no intentional deviation.
The following cell line samples were obtained from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research: GM24385
The dataset comprises multiple flowcells for each pore:
Location of the data
The data is located in an Amazon Webservice S3 bucket at:
See the tutorials page for information on downloading the dataset.
Description of available data
The uploaded dataset has been prepared using a snakemake pipeline to:
For more details see our post detailing the pipeline and its outputs.