The data referenced in this page have been superceded by the June 2021 Guppy 5 rebasecalling
We are happy to annouce an updated release of nanopore sequencing of the Genome in a Bottle sample GM24385.
Multiple PromethION flowcells using R9.4.1 nanopores were used, along with two different sample preparation methods. The direct sequencer output is included, raw signal data stored in .fast5 files and basecalled data in .fastq file. Additional secondary analyses are included, notably alignments of sequence data to the reference genome are provided along with statistics derived from these.
Highlights of the dataset include:
A brief report comparing the current release to the previous can be found here.
The dataset comprises data from multiple R9.4.1 flowcells.
The initial sequencer outputs are included in self container directories. In addition derived outputs from an automated pipeline are stored separately.
Details concerning sample preparations
Below is described briefly the method of analyte preparation. Standard, published protocols were followed with no intentional deviation.
The following cell line samples were obtained from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research: GM24385
The extracted DNA was subjected to one of two protocols. The first protocol entailed:
The second protocol used the new Ultralong Sequencing Kit (ULK).
In both cases sequencing was performed using PromethION device.
The dataset comprises multiple flowcells for each pore:
Location of the data
The data is located in an Amazon Web Services S3 bucket at:
See the tutorials page for information on downloading the dataset.
Description of available data
The uploaded dataset has been prepared using a snakemake pipeline to:
For more details see our post detailing the pipeline and its outputs.