wf-mpx documentation
wf-mpx | Mpox Virus Metagenomic Assembly
This repository contains a nextflow workflow for the assembly of reads originating from the mpox virus obtained through Oxford Nanopore metagenomic sequencing.
Introduction
This workflow provides a simple way to analyse mpox sequencing data; taking raw Oxford Nanopore Technologies reads and creating a draft consensus and assembly.
We expect data to be generated from a mixed population sample hence the use of the term “metagenomics”.
No trimming of sequences is carried out so be vigilant when using targeted data.
Using community-develped tools, this workflow:
- Maps reads to a reference genome (
minimap2) - Assesses coverage
- Discards reads not mapping to the chosen reference
- Calls variants with resepect to the reference (
medaka) - Filters variants with <20x coverage
- Creates a draft consensus (
bcftools) - Creats a de-novo assembly (
flye&medaka)
More information can be found in this blog post.
Quickstart
The workflow uses nextflow to manage compute and software resources, as such nextflow will need to be installed before attempting to run the workflow.
The workflow can currently be run using either Docker or singularity to provide isolation of the required software. Both methods are automated out-of-the-box provided either docker or singularity is installed.
It is not required to clone or download the git repository in order to run the workflow. For more information on running EPI2ME Labs workflows visit out website.
Workflow options
To obtain the workflow, having installed nextflow, users can run:
nextflow run epi2me-labs/wf-mpx --help
to see the options for the workflow.
Workflow example
To run the workflow with test data
git clone https://github.com/epi2me-labs/wf-mpxnextflow run epi2me-labs/wf-mpx --fastq wf-mpx/test_data/fastq/barcode01
Workflow outputs
The primary outputs of the workflow include:
- a simple text file providing a summary of sequencing reads,
- an HTML report document detailing the primary findings of the workflow.
- a draft consensus sequence obtained
- a medaka polished assembly
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